2001 Lyme borreliosis in rhesus macaques-effects of
corticosteroids on spirochetal load and isotype switching of anti-borrelia
Lyme borreliosis in rhesus macaques: chronic Ld.
6:31:18 PM Eastern Standard Time
Lyme borreliosis in rhesus macaques: effects of corticosteroids on spirochetal
load and isotype switching of anti-borrelia burgdorferi antibody.
Clin Diagn Lab Immunol 2001 Mar;8(2):225-32 (ISSN: 1071-412X)
Pachner AR; Amemiya K; Bartlett M; Schaefer H; Reddy K; Zhang WF [Find other
with these Authors]
Department of Neurosciences, University of Medicine and Dentistry of New
Jersey-New Jersey Medical School, 185 S. Orange St., Newark, NJ 07103, USA.
Experimental Borrelia burgdorferi infection of rhesus monkeys is an excellent
model of Lyme disease and closely parallels the infection in humans. Little is
known about the interaction of host immunity with the spirochete in patients
with chronic infection. We hypothesized that rapid development of anti-B.
burgdorferi antibody in immunocompetent nonhuman primates (NHPs) is the
major determinant of the reduction of the spirochetal load in Lyme borreliosis.
This hypothesis was tested by measurement of the spirochetal load by PCR in
association with characterization of the anti-B. burgdorferi humoral immune
response in immunocompetent NHPs versus that in corticosteroid-treated NHPs.
Although anti-B. burgdorferi immunoglobulin G (IgG) antibody was effectively
inhibited in dexamethason (Dex)-treated NHPs, anti-B. burgdorferi IgM antibody
levels continued to rise after the first month and reached levels in excess of
IgM levels in immunocompetent NHPs. This vigorous production of anti-B.
burgdorferi IgM antibodies was also studied in vitro by measurement of antibody
produced by B. burgdorferi-stimulated peripheral blood mononuclear cells.
Despite these high IgM antispirochetal antibodies in Dex-treated NHPs,
spirochetal loads were much higher in these animals. These data indicate that
Dex treatment results in interference with isotype switching in this model and
provide evidence that anti-B. burgdorferi IgG antibody is much more effective
than IgM antibody in decreasing the spirochetal load in infected animals.
Major Subject Heading(s) Minor Subject Heading(s) CAS Registry / EC Numbers
Borrelia burgdorferi [immunology]
Glucocorticoids, Synthetic [pharmacology]
Immunoglobulin Class Switching [immunology]
Lyme Neuroborreliosis [immunology]
Find other articles with similar Subjects.
Antibodies, Bacterial [blood] [cerebrospinal fluid]
Antibody Formation [immunology]
Disease Models, Animal
IgG [blood] [cerebrospinal fluid]
IgM [blood] [cerebrospinal fluid]
Immunoglobulin Class Switching [drug effects]
0 (Antibodies, Bacterial)
0 (Glucocorticoids, Synthetic)
Indexing Check Tags: Animal; Female; Male
MEDLINE Indexing Date: 200106
Publication Type: Journal Article
PreMedline Identifier: 0011238200
Unique NLM Identifier: 21137938
Journal Code: IM
6:31:18 PM Eastern Standard Tim
In a message dated
11:27:54 AM Eastern Standard Time, barb writes:
(she was a dear Lyme friend) offered
the following as proof of Borrelia persistence. These emails were
originally posted on Actionlyme and also sent privately.